Temporary

Part:BBa_J70562:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-02-17)


C-Terminal fusion test: J70561:J70550


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1738
    Illegal AgeI site found at 1850
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

assembled


Source

digest with BsaXI and insert this to add a C-terminal tag

forward (J70562-f): aaGGATCCCACCACCACCACCACCACTAATAAtactagtagcggc

reverse (J70562-r): gctactagtaTTATTAGTGGTGGTGGTGGTGGTGGGATCCtttgt

References